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JCAnderson 11:34, 13 March 2013 (EDT)

EIPCR primers for making a 6xHis_tADH1 "Part 4" part plasmid

sbb13-1F: GCATCGTCTCACATCATCATCATCATCACtaactcgaggcgaatttc sbb13-2R: ATGCCGTCTCAGATGggatggagaccgactacc template: pGG062

PCR product should be 1947bp. Cut with BsmBI, ligate, plate on LB+Cam (ColE1 origin). Then when you assemble an expression plasmid, you can either use pGG062 to add the stop codon+terminator, or this new plasmid to add a 6xHis+stop+terminator.

JCAnderson 22:16, 31 January 2012 (EST)

This is my next entry.