SBB09 Construct6

Construction of upaG basic part
PCR Ost001F and Ost002R on CFT073 genomic DNA      (298bp (282+16), EcoRI/BamHI)
Sub into JCA_pBca9495KC-Bca1144                    (EcoRI/BamHI, 2974+910, L)
Product is JCA_pBca9495KC-M10026     {upaG}
----------------------------
Ost001F   Cloning of upaG   ccaaaGAATTCatgAGATCTgttgagatggataacaaactg
Ost002R   Cloning of upaG   ctagcGGATCCttaCCACtgaataccggcaccgag

 JCA short description renamed

Construction of upaG basic part
PCR Ost001F and Ost002R on CFT073 genomic DNA      (298bp (282+16), EcoRI/BamHI)
Sub into JCA_pBca9495KC-Bca1144                    (EcoRI/BamHI, 2974+910, L)
Product is JCA_pBca9495KC-M10026     {<upaG_short!}
----------------------------
Ost001F   Cloning of upaG   ccaaaGAATTCatgAGATCTgttgagatggataacaaactg
Ost002R   Cloning of upaG   ctagcGGATCCttaCCACtgaataccggcaccgag