SBB09 Construct46
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Construction of mini protein A Z domain mutant
Wobble Ojw001 / Ojw002 (133bp, EcoRI/BamHI)
Sub into pBca9495AK-Bca1144#5 (EcoRI/BamHI, L)
Product is pBca9495AK-M10018 {<Z34C domain>}
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Ojw001 Forward construction of Z34C basic part
CCATAgaattcATGagatctTTCAACATGCAGTGCCAGCGTCGTTTCTACGAAGCTCTGCACGACCCGAACCTGAACGAAG
Ojw002 Reverse construction of Z34C basic part
CTGATggatccGCAGTCGTCACGGATAGATTTGATTTTAGCGTTACGCTGTTCTTCGTTCAGGTTCGGGTCG
