SBB09 Construct44
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Construction of Polystyrene-Binding Peptide
Wobble Ons003F/Ons004R (67bp, EcoRI/BamHI)
Sub into pBca9495AK-Bca1144#5 (EcoRI/BamHI, 3171+910, L)
Product is pBca9495AK-M10021 {<Poly_bind>}
----
Ons003F Forward construction of Poly_bind basic part ccataGAATTCatgAGATCTttcaaattctggctatacgaacatgtaatacggggg
Ons004R Reverse construction of Poly_bind basic part ctgatGGATCCcccccgtattacatgttcgt
JCA: Your construction file is "correct", but in general repeats on the 3' end of an oligo are bad news, particularly if they are G's since 4G's makes a particular secondary structure in and of itself. See revision:
Construction of Polystyrene-Binding Peptide
Wobble Ons003F/Ons004R (67bp, EcoRI/BamHI)
Sub into pBca9495AK-Bca1144#5 (EcoRI/BamHI, 3171+910, L)
Product is pBca9495AK-M10021 {<Poly_bind>}
----
Ons003F Forward construction of Poly_bind basic part ccataGAATTCatgAGATCTttcaaattctggctatacgaacatg
Ons004R Reverse construction of Poly_bind basic part ctgatGGATCCcccccgtattacatgttcgtatagccagaat
