SBB09 Construct43
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Construction of {<TraT>} part
PCR Ons001F/Ons002R on TraT (342 bp, BamHI/EcoRI)
Digest pBca9495CA-Bca1144#5 (BamHI/EcoRI, 3039+910, L)
Product is pBca9495-M10020 {<TraT>}
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Ons001F Forward BglII for TraT ccataGAATTCatgAGATCTaaaccccgtaaacagcattcgcctg
Ons002R Reverse BamH1 for TraT ctgatGGATCCggccaaatcaatcgcaaatattctcccccccgaaCtcaggc
JCA: I think you got the wrong template sequence somehow here. See revision below:
Construction of {<TraT>} part
PCR Ons001F/Ons002R on TraT (700 bp, BamHI/EcoRI)
Digest pBca9495CA-Bca1144#5 (BamHI/EcoRI, 3039+910, L)
Product is pBca9495CA-M10020 {<TraT>}
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Ons001F Forward oligo for TraT
ccataGAATTCatgAGATCTtgtggtgcgatgagcacagc
Ons002R Reverse oligo for TraT
ctgatGGATCCgagaatatttgcgattgatttgg
