SBB09 Construct42
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Construction of Ice Nucleation Repeats (INP repeats) Basic Part
PCR Oeh001F/Oeh002R on pBca1256-Bca1346 {2296bp, EcoRI/BamHI}
Sub into pBca9145-Bca1144#5 {EcoRI/BamHI, 3171+910,L}
Product is pBca9145-Beh001 {<INP repeats>}
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eh001F Forward EcoRI oligo for <INP repeats>
gtcagGAATTCatgAGATCTaaccacagtgatctaattgc
eh001R Reverse BamHI oligo for <INP repeats>
gtcagGGATCCactgtcctgcccggccgttg
JCA: the pcr is right, but you have it in the wrong vector. Also, use your M numbers. See revised:
Construction of Ice Nucleation Repeats (INP repeats) Basic Part
PCR Oeh001F/Oeh002R on pBca1256-Bca1346 (2296bp, EcoRI/BamHI)
Sub into pBca9495AK-Bca1144#5 (EcoRI/BamHI, L)
Product is pBca9495AK-M10048 {<INP repeats>}
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eh001F Forward EcoRI oligo for <INP repeats>
gtcagGAATTCatgAGATCTaaccacagtgatctaattgc
eh001R Reverse BamHI oligo for <INP repeats>
gtcagGGATCCactgtcctgcccggccgttg
