SBB09 Construct39

Construction of <a~Inv-long> basic part Bcr0002

PCR Ocr0001-F/Ocr0002-R on pAC-TetInvdBg (2749 bp, EcoRI/BamHI)
Sub into pBca9495CA-Bca1144#5 (EcoRI/BamHI, 3039+910, L)
Product is pBca9495CA-Bcr0002  {a~Inv-long>}
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Ocr0001-F       forward oligo for biobricking of {a~Inv-long>}
ccaaaGAATTCatgAGATCTaattatgatggttttccagccaatcagtg
Ocr0002-R       reverse oligo for biobricking of  {a~Inv-long>}
ccaaaGGATCCctgctggcctcAagactggatacc

 JCA:  Use Douglas's Odmd002R for your reverse; you need to use the M numbers for naming

Construction of <a~Inv-long> basic part Bcr0002

PCR Ocr0001-F/Odmd002R on pAC-TetInvdBg (2749 bp, EcoRI/BamHI)
Sub into pBca9495CA-Bca1144#5 (EcoRI/BamHI, 3039+910, L)
Product is pBca9495CA-M10031  {a~Inv-long>}
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Ocr0001-F       forward oligo for biobricking of {a~Inv-long>}
ccaaaGAATTCatgAGATCTaattatgatggttttccagccaatcagtg
Odmd002R	Reverse Oligo for Invasin (refactored, long)
ccaaaGGATCCactggataccagcgatctgccg