SBB09 Construct37
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Construction of {<KILR>} basic part
Wobble Oph024-F/Oph025-R (136 bp, EcoRI/BamHI)
Sub into pBca9495CA-Bca1144#5 (EcoRI/BamHI, 3039+910, L)
Product is pBca9495CA-M10037 {<KILR>}
----
Oph024-F foward oligo for cloning of {<KILR>}
ccataGAATTCatgAGATCTgttaaagaactggaagacaaaaacgaagaactgctgtctaaaatctaccacctgcg
Oph025-R reverse oligo for cloning of {<KILR>}
ctgatGGATCCgcaaccaccacgttcaccaaccagttttttcagacgagcaacttcgttacgcaggtggtagattttagacagc
JCA: wrong vector. See revision below:
Construction of {<KILR>} basic part
Wobble Oph024-F/Oph025-R (136 bp, EcoRI/BamHI)
Sub into pBca9495AK-Bca1144#5 (EcoRI/BamHI, L)
Product is pBca9495AK-M10037 {<KILR>}
----
Oph024-F foward oligo for cloning of {<KILR>}
ccataGAATTCatgAGATCTgttaaagaactggaagacaaaaacgaagaactgctgtctaaaatctaccacctgcg
Oph025-R reverse oligo for cloning of {<KILR>}
ctgatGGATCCgcaaccaccacgttcaccaaccagttttttcagacgagcaacttcgttacgcaggtggtagattttagacagc
