SBB09 Construct33
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Wobble OWRL-025-F/OWRL-026-R (67bp, EcoRI/BamHI)
Sub into pBca9145AK-Bca1144#5 (EcoRI/BamHI, 3071+910, L)
Product is pBca9145AK-M10033 {<Aluminum Binding Peptide>}
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OWRL-025-F Forward construction of Aluminum Binding Peptide basic part
GCGTAgaattcATGagatctGTGCCGAGCAGCGGCCCGCAGGATACC
OWRL-026-R Reverse construction of Aluminum Binding Peptide basic part
TCATCggatccGGTGGTGCGGGTATCCTGCGGGCCGCTGC
JCA: Wrong vector, see revision:
Wobble OWRL-025-F/OWRL-026-R (67bp, EcoRI/BamHI)
Sub into pBca9495AK-Bca1144#5 (EcoRI/BamHI, 3071+910, L)
Product is pBca9495AK-M10033 {<Aluminum Binding Peptide>}
----
OWRL-025-F Forward construction of Aluminum Binding Peptide basic part
GCGTAgaattcATGagatctGTGCCGAGCAGCGGCCCGCAGGATACC
OWRL-026-R Reverse construction of Aluminum Binding Peptide basic part
TCATCggatccGGTGGTGCGGGTATCCTGCGGGCCGCTGC
