SBB09 Construct32
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Biobricking of Intimin EaeA
PCR OWRL-023-F/OWRL-024-R on {<Intimin EaeA>} (1887bp, EcoRI/BamHI)
Sub into pBca9495CA-Bca1144#5 (EcoRI/BamHI, 910+3039,L)
Product is pBca9495CA-M10032
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OWRL-023-F Forward biobricking of Intimin EaeA
GCGTAgaattcATGagatctAATGGTGAAAATTATTTTAAATTGGGTTCGG
OWRL-024-R Reverse biobricking of Intimin EaeA
GCAAAggatccGCCTTGGTTTGATCAAAAAATATAACCGCAC
JCA: Reverse oligo can be reused. See revision:
Biobricking of Intimin EaeA
PCR OWRL-023-F/Bhs003R on {<Intimin EaeA>} (1888 bp, EcoRI/BamHI)
Sub into pBca9495CA-Bca1144#5 (EcoRI/BamHI, 910+3039,L)
Product is pBca9495CA-M10032 {<eaeA refactored>}
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OWRL-023-F Forward biobricking of Intimin EaeA
GCGTAgaattcATGagatctAATGGTGAAAATTATTTTAAATTGGGTTCGG
Bhs003R Reverse BamHI for a~eaeA_Display>
GCAAAggatccGGCCTTGGTTTGATCAAAAAATATAACCGCAC
