SBB09 Construct3

Bglbricking of M10034, Autotransporter
PCR  Oso001 and Oso002 on E. coli MG1655 genomic DNA (961 bp, EcoRI/BamHI)
Sub into pBca9145KC-Bca1144#5    (EcoRI/BamHI, 910+2974, L)
Product is pBca9145KC-M10034    {<Autotransporter!}
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 Oso001 Cloning of Autotransporter  ccataGAATTCatgAGATCTgaagtccccctgtatgcctc
 Oso002 Cloning of Autotransporter  GCTAGggatccTCAataaccggcctgaacgcc

 JCA Note:  The start and stop positions for this construction file aren't quite right.
 See revised construction file below

Bglbricking of M10034, Ag43 (autotransporter only)
PCR  Oso001 and Oso002 on E. coli MG1655 genomic DNA (937 bp, EcoRI/BamHI)
Sub into pBca9495KC-Bca1144#5    (EcoRI/BamHI, L)
Product is pBca9495KC-M10034    {<Ag43_short!}

 Oso001 Cloning of Ag43, short ccataGAATTCatgAGATCTggtgaaaacaacagcgtccg
 Oso002 Cloning of Ag43 GCttaggatcctcagaaggtcacattcagtg