SBB09 Construct21
From OpenWetWare
Jump to navigationJump to search
Wobble Osc0028F/Osc0029R (136bp, EcoRI/BamHI)
Sub into pBca9495AK-Bca1144#5 (EcoRI/BamHI, 3171+910, L)
Product is pBca9495AK-M10053 {passenger protein}
----
Osc0028F Forward construction of passenger protein basic part
TCCGGgaattcATTagatctGTTAAAGAACTGGAAGACAAAAACGAAGAACTGCTGTCTGAAATCTACCACCTGGACAACGAAG
Osc0029R Reverse construction of passenger protein basic part
CTGGTggatccGCAACCACCACGTTCACCAACCAGTTTTTTCAGACGAGCAACTTCGTTGTCCAGGTGGTAG
JCA: Put atg between eco and bam; renamed short description. Revised below:
Wobble Osc0028F/Osc0029R (136bp, EcoRI/BamHI)
Sub into pBca9495AK-Bca1144#5 (EcoRI/BamHI, 3171+910, L)
Product is pBca9495AK-M10053 {<EILD>}
----
Osc0028F Forward construction of Leucine zipper EILD
TCCGGgaattcATGagatctGTTAAAGAACTGGAAGACAAAAACGAAGAACTGCTGTCTGAAATCTACCACCTGGACAACGAAG
Osc0029R Reverse construction of Leucine zipper EILD
CTGGTggatccGCAACCACCACGTTCACCAACCAGTTTTTTCAGACGAGCAACTTCGTTGTCCAGGTGGTAG