SBB09 Construct20
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Construction of ehaB Basic Part
PCR Osc0030F/Osc0031R on ehaB gene (1257bp, EcoRI/BamHI)
Sub into pBca9495KC-Bca1144#5 (EcoRI/BamHI, 2974+910, L)
Product is pBca9495KC-M10052 {<ehaB!]
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Osc0030F Forward Biobricking of ehaB
ggcctGAATTCattAGATCTggcttaatcgcaatgaactct
Osc0031R Reverse Biobricking of ehaB
gtaccGGATCCttaccaggtatatttaacac
JCA: This might go past the "true" stop, but should still work either way
