SBB09 Construct10
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Bglbricking of espP(beta)
PCR osn0001/osn0002 on Escherichia coli O157:H7 str. Sakai (1381 bp, EcoRI/BamHI)
Sub into pBca9495KC-Bca1144 (EcoRI/BamHI, 2974+910, L)
Product is pBca9495KC-M10044 {<espP(beta)!}
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Osn0001-F Forward Bglbricking of espP of Escherichia coli O157:H7 str. Sakai ccagtGAATTCatgAGATCTgccccgtcagcatctgccac
Osn0002-R Reverse Bglbricking of espP of Escherichia coli O157:H7 str. Sakai gcagtGGATCCtcagaacgagtaacggaaattag