SBB09 Construct1
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PCR ca998/ca1034 on pSB1A2-Bca9033 (~350 bp, EcoRI/BamHI)
Sub into pBca9145-Bca1089 (EcoRI/BamHI, Large)
Product is pBca1020-Bca1034 {fdhF2}
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ca1034 Reverse biobrick oligo for fdhF
CACTTggatccAATAGGGGCAAACCGTGACGAC
PCR ca9394/ca9395 on colE2 (1758 bp, BglII/XhoI)
Sub into pBca9145-Bca1089 (BglII/XhoI, 2057+696, L)
Product is pBca9145-Bca9394 {ceaB}
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ca9394 Cloning of ceaB ccaaaAGATCTatgagcggtggcgatggacg
ca9395 Cloning of ceaB gctagCTCGAGttaGGATCCtcaatatgtcgcttaggtgt
