S. cerevisiae genotypes

Common yeast strains

van Dijken00 describes the characteristics of many common laboratory strains.

S288C

MATα SUC2 gal2 mal mel flo1 flo8-1 hap1

• gal2^-; does not use galactose anaerobically
• does not form pseudohyphae
• ATCC: 204508
• Mortimer86

AB1380

MATa ura3-52 trp1-289 lys2-1 ade2-1 can1-100 his5 ρ⁺ ψ⁺

• ATCC 204682, 20843, 201447
• AB1380 is MATa not MATα in contradiction to the article in Science 236: 806-812, 1987
• Burke87

J57D

MATa ura3-52 trp1 ade2-101 can1-100 leu2-3,112 his3-2,15

• used in Haldi96 for the mouse genome project
• from Vladimir Larionov
• Zhong98 claims MATα and his3-6
• several times better transformation efficiency than AB1380

W303-1a

MATa leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15 ybp1-1

• Open Biosystems: YSC1058
• Rothstein notes
• Veal03

VL6-48N

MATα trp1-Δ1 ura3-Δ1 ade2-101 his3-Δ200 lys2 met14 cir°

• high transformation efficiency strain for spheroplast YAC construction
• Vladimir Larionov papers on TAR (transformation associated recombination)
• generated from VL6-48 by substitution of the KanMX cassette into ura3-52
• Construction: Kouprina98 (from VL6-48); Larionov96 (from YPH857, Sikorski89, Larionov94)

YPH499

MATa ura3-52 lys2-801_amber ade2-101_ochre trp1-Δ63 his1-Δ200 leu2-Δ1

• derived from S288C
• contains deletions of most selectable markers
• retains the promoter for the trp gene, thus homology with that plasmid sequence
• gal2^-; does not use galactose anaerobically
• ATCC:204679
• Sikorski89, Sobel99

YPH500

MATα ura3-52 lys2-801_amber ade2-101_ochre trp1-Δ63 his1-Δ200 leu2-Δ1

• derived from S288C
• contains deletions of most selectable markers
• retains the promoter for the trp gene, thus homology with that plasmid sequence
• gal2^-; does not use galactose anaerobically
• ATCC:204680
• Sikorski89, Sobel99

Window Strains

• Strains are derivative of YPH925

• Strains have gaps in the chromosome size range to allow gel extraction of YACs with specific sizes. See Hamer95.

• PFG conditions are BioRad Chef 1% SeaPlaque GTG agarose at 6 V/cm 0.5x TBE 12 C

• YACs can be transfered to these strains by kar1^- mating, see Hugerat94 and Spencer94.

YBLW1A

MATα leu2-Δ1 trp1-Δ63 ura3-52 ade2-101 his3-Δ200 lys2-801 cyh2^R kar1-Δ15 cys3

• Window 150-280 Kbp
• PFG 15-25s, 24 hrs
• Genotype is listed for YBLW1; YBLW1A may have differences, but is the strain we have.

YBLW2

MATα leu2-Δ1 trp1-Δ63 ura3-52 ade2-101 his3-Δ200 lys2-801 cyh2^R kar1-Δ15 met10 thr4

• Window 250-450 Kbp
• PFG 20-35s, 24 hrs

YBLW3

MATα leu2-Δ1 trp1-Δ63 ura3-52 ade2-101 his3-Δ200 lys2-801 cyh2^R kar1-Δ15 met10 thr4

• Window 310-590 Kbp
• PFG 35-60s, 24 hrs

YBLW4

MATα leu2-Δ1 trp1-Δ63 ura3-52 ade2-101 his3-Δ200 lys2-801 cyh2^R kar1-Δ15 thr1

• Window 450-680 Kbp
• PFG 50-70s, 24 hrs

YBLW5

MATα leu2-Δ1 trp1-Δ63 ura3-52 ade2-101 his3-Δ200 lys2-801 cyh2^R kar1-Δ15 met14

• Window 590-755 Kbp
• PFG 50-80s, 36 hrs

YBLW6

MATα leu2-Δ1 trp1-Δ63 ura3-52 ade2-101 his3-Δ200 lys2-801 cyh2^R kar1-Δ15

• Window 680-950 Kbp
• PFG 60-100s, 36 hrs

YBLW7

MATα leu2-Δ1 trp1-Δ63 ura3-52 ade2-101 his3-Δ200 lys2-801 cyh2^R kar1-Δ15

• Window 810-1120 Kbp
• PFG 70-120s, 48 hrs

YBLW8

MATα leu2-Δ1 trp1-Δ63 ura3-52 ade2-101 his3-Δ200 lys2-801 cyh2^R kar1-Δ15

• Window 985-1640 Kbp
• PFG 80-130s, 48 hrs

YBLW9

MATα leu2-Δ1 trp1-Δ63 ura3-52 ade2-101 his3-Δ200 lys2-801 cyh2^R kar1-Δ15

• Window 1140-2000 Kbp (upper range varies due to the presence of ribosomal repeats in chromosome XII)
• PFG 100-160s, 48 hrs

References

1. Mortimer RK and Johnston JR. Genealogy of principal strains of the yeast genetic stock center. Genetics. 1986 May;113(1):35-43. DOI:10.1093/genetics/113.1.35 |
2. Burke DT, Carle GF, and Olson MV. Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors. Science. 1987 May 15;236(4803):806-12. DOI:10.1126/science.3033825 |
3. Sikorski RS and Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics. 1989 May;122(1):19-27. DOI:10.1093/genetics/122.1.19 |
4. Hugerat Y, Spencer F, Zenvirth D, and Simchen G. A versatile method for efficient YAC transfer between any two strains. Genomics. 1994 Jul 1;22(1):108-17. DOI:10.1006/geno.1994.1351 |
5. Spencer F, Hugerat Y, Simchen G, Hurko O, Connelly C, and Hieter P. Yeast kar1 mutants provide an effective method for YAC transfer to new hosts. Genomics. 1994 Jul 1;22(1):118-26. DOI:10.1006/geno.1994.1352 |
6. Larionov V, Kouprina N, Nikolaishvili N, and Resnick MA. Recombination during transformation as a source of chimeric mammalian artificial chromosomes in yeast (YACs). Nucleic Acids Res. 1994 Oct 11;22(20):4154-62. DOI:10.1093/nar/22.20.4154 |
7. Hamer L, Johnston M, and Green ED. Isolation of yeast artificial chromosomes free of endogenous yeast chromosomes: construction of alternate hosts with defined karyotypic alterations. Proc Natl Acad Sci U S A. 1995 Dec 5;92(25):11706-10. DOI:10.1073/pnas.92.25.11706 |
8. Kouprina N, Annab L, Graves J, Afshari C, Barrett JC, Resnick MA, and Larionov V. Functional copies of a human gene can be directly isolated by transformation-associated recombination cloning with a small 3' end target sequence. Proc Natl Acad Sci U S A. 1998 Apr 14;95(8):4469-74. DOI:10.1073/pnas.95.8.4469 |
9. Sobel SG and Wolin SL. Two yeast La motif-containing proteins are RNA-binding proteins that associate with polyribosomes. Mol Biol Cell. 1999 Nov;10(11):3849-62. DOI:10.1091/mbc.10.11.3849 |
10. van Dijken JP, Bauer J, Brambilla L, Duboc P, Francois JM, Gancedo C, Giuseppin ML, Heijnen JJ, Hoare M, Lange HC, Madden EA, Niederberger P, Nielsen J, Parrou JL, Petit T, Porro D, Reuss M, van Riel N, Rizzi M, Steensma HY, Verrips CT, Vindeløv J, and Pronk JT. An interlaboratory comparison of physiological and genetic properties of four Saccharomyces cerevisiae strains. Enzyme Microb Technol. 2000 Jun 1;26(9-10):706-714. DOI:10.1016/s0141-0229(00)00162-9 |
11. Veal EA, Ross SJ, Malakasi P, Peacock E, and Morgan BA. Ybp1 is required for the hydrogen peroxide-induced oxidation of the Yap1 transcription factor. J Biol Chem. 2003 Aug 15;278(33):30896-904. DOI:10.1074/jbc.M303542200 |