RobWarden:M13Redo
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M13K07 Phage Genome Renovation Ideas
Proposed Changes
- Put genes I, IV, XI under a stronger promoter
- Create more pores for phage to be extruded
- Too many pores could be harmful to host cell
Summary of Changes
| Gene | Function | Renovation Ideas |
|---|---|---|
| I | Assembly (inner membrane) | Stronger promoter |
| II | Replication of + ssDNA | |
| III | Tail (interacts with F pilus) | |
| IV | Assembly (outer membrane) | Stronger promoter |
| V | Binds ssDNA | |
| VI | Tail (buried under III) | |
| VII | Head (buried under IX) | |
| VIII | Coat | |
| IX | Head (exposed) | |
| X | DNA Replication | |
| XI | Assembly (inner membrane) | Stronger Promoter |
