Rich Media

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Materials

1. 11 g yeast extract

2. 22 g bacto-peptone

3. 55 mg adenine

4. About 900 ml distilled water

5. 100 ml 20% autoclaved glucose solution

6. 1 liter container


Procedure

1. Combine 1-3 in 6

2. Add 4 until total volume is 900ml

3. Mix well, and autoclave. Solution should be a transparent brown.

4. Add 5, and mix well

Wait for solution to cool to room temperature before using