Restriction digest/Buffers

New England Biolabs

1X NEBuffer 1

• 10 mM Bis-Tris-Propane-HCl
• 10 mM MgCl₂
• 1 mM Dithiothreitol
• pH 7.0 @ 25°C

1X NEBuffer 2

• 50 mM NaCl
• 10 mM Tris-HCl
• 10 mM MgCl₂
• 1 mM Dithiothreitol
• pH 7.9 @ 25°C

1X NEBuffer 3

• 100 mM NaCl
• 50 mM Tris-HCl
• 10 mM MgCl₂
• 1 mM Dithiothreitol
• pH 7.9 @ 25°C

1X NEBuffer 4

• 50 mM potassium acetate
• 20 mM Tris-acetate
• 10 mM Magnesium Acetate
• 1 mM Dithiothreitol
• pH 7.9 @ 25°C