Reshma Shetty/Endy group meeting
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| Construct | Purpose | Constructed | Sequence verified | Tested |
| pSB1AC3-R0040.B0030.p53ZFF1F2.JunD.B0015 | To better control for load effects. | Yes | Yes | No |
| BBa_C2004 | Codon-optimized repressor which binds to 12bp operator | Yes (pSB4A3-R0040.B0030.C2004.B0015 colony 2 and pCR4-TOPO-B0030.C2004.B0015 colony 1) | Yes | No |
| BBa_C2005 | Repressor without His tag | Yes (pCR4-TOPO-B0030.C2005 colony 16) | Yes | No |
| BBa_C2006 | Repressor with N-terminal MBP | Failed, repeating | No | No |
| BBa_C2007 | Repressor with C-terminal MBP | Failed, repeating | No | No |
| BBa_R2109 | Promoter with optimally placed 12bp operator site | Yes (pSB4A3-R2109 colony 3) | Yes | No |
| BBa_R2114 | Promoter with near optimally placed 12bp operator site | Yes | Yes | No |
| New promoter series | Promoters with 14bp operator. | To be designed. | ||
Does anyone have problems amplifying R0040? Seemed to work when cloned into low copy plasmids.
