M/N Digest of I2055
- Hopefully it has the promoter
- Ligated into 1AK3 and transformed.
- Running a gel
- loading order:T9002 in 3K3 (4-1), I2055 in 1AK3 (1-4), E0240 in 3K3(4-1), E0240 in 1AK3(1,2).
- Seems to be a problem with 3K3 (perhaps not digested fully?)
- Making cultures to sequence:
- T9002-3K3(bad): #3
- I2055-1AK3: #1
- E0240-3K#(bad): #3
- E0240-1AK3: #1
- Since 3K3 may be causing the problems, making o/n of it to mini-prep tomorrow and digest.
- Prepared sequencing reaction.