Registry/Measurement kit/Notebook/2007-7-7

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  • Got growth on:
    • T9002
      • 3K3 (from both sequential and double digests)
    • I2055
      • 1AK3
    • E0240
      • 3K3
      • 1AK3 (2 colonies)
  • Will colony PCR
  • Gel of I2055 (with promoter) looks ok, will sequence to make sure.
  • PCR cleaned I2055
  • Double M/N digest in buffer 2
  • I2056 doesn't seem to grow in o.n. amp/tet culture...trying with just tet.

  • Ligating T9002 into 1AK3