- running 10uL of PCR on a small gel
- Sent samples to be sequenced
- Did PCR purification on MfeI digest, but DNA is very dilute: 13.1 ng/uL
- Trying double digest in parallel with NsiI digest
- Primers arrived to insert BB sites. See 7/2.
- Diluting primers, starting PCR on E0240. Tm = 53.5