Registry/Measurement kit/Notebook/2007-7-28

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Overnight cultures

  • Growth on:
    • E0240-1AK3
    • I2055-1AK3
    • I2057-1AK3
    • I2056-1AO3
    • Both promoter testers (3K3, ligated at NotI) versions E-X and E-S
  • None:
    • F2620-1AK3 and 3K3
    • Replate of F2620-1AK3

I2055-1AK3

  • Ran gel of last night's PCR, all colonies were wrong size.


Colony PCRs

  • Gel images:
7-28-lrg.jpg
  • Large gel:
    • I2057-ES (2,1); I2057-EX (6-1)
    • E0240-ES(4-1); I2057-ES(6-3)
7-28-07-sm.jpg
  • Small gel:
    • E0240-EX (6-1); E0240-ES(6,5)
    • I2056-1AK3 (2,1); I2057-1AK3 (2,1); E0240-1AK3 (1); I2055-1AK3(3-1)


  • Made overnights of:
    • I2057-EX #6
    • I2057-ES #4
    • E0240-EX #1
    • E0240-ES #2
    • E0240-1AK3 #1 (not biobricked)

Transformations

  • Ligated ccdB into all promoter testers and transformed.