Registry/Measurement kit/Notebook/2007-7-28

Overnight cultures

• Growth on:
  • E0240-1AK3
  • I2055-1AK3
  • I2057-1AK3
  • I2056-1AO3
  • Both promoter testers (3K3, ligated at NotI) versions E-X and E-S
• None:
  • F2620-1AK3 and 3K3
  • Replate of F2620-1AK3

I2055-1AK3

• Ran gel of last night's PCR, all colonies were wrong size.

Colony PCRs

• Gel images:

• Large gel:
  • I2057-ES (2,1); I2057-EX (6-1)
  • E0240-ES(4-1); I2057-ES(6-3)

• Small gel:
  • E0240-EX (6-1); E0240-ES(6,5)
  • I2056-1AK3 (2,1); I2057-1AK3 (2,1); E0240-1AK3 (1); I2055-1AK3(3-1)

• Made overnights of:
  • I2057-EX #6
  • I2057-ES #4
  • E0240-EX #1
  • E0240-ES #2
  • E0240-1AK3 #1 (not biobricked)

Transformations

• Ligated ccdB into all promoter testers and transformed.