- No growth on I2056.
- F2620-3K3 sample 1 and F2620-1AK3 have a few small colonies. F2620-3K3 sample 2 has 1 colony
- PCR clean digests from yesterday
- Ligate them
- Mini-Prep and digest 3K3 (E/X)
- PCR products are digested and cleaned up.
- Ran gel of PCR, made o/n of only colony that looked right size.
- Running pcr of F2620-3k3 and 1AK3
- Primers came today, so started E0240 (E-S) and I2057 (E-X) (Tm=52 deg)
- I2055-1AK3 and 3K3
- I2056-1AK3 and 3K3
- Decided not to make the silent point mutation; will use an AatII site on 3K3 (~100bp upstream of the cloning site) instead of MfeI