Registry/Measurement kit/Notebook/2007-7-19

Primers

• Creating new E0240 reverse primer for T9002: (NsiI)
  • TCAGCGAT ATGCAT TATAAACGCAGAAAGGCCCAC

• Yesterday's PCR didn't work, as expected.
• Made new ladder dilution:
  • 1µL ladder
  • 9µL water
  • 2µL loading buff

Sequencing results

• Scarred: E0240 (9), I2057 (2, 4)
• To be trashed: I2055 (3,4,16), E0240 (1,2)

I2055

• Trying to add R0040 to I2055 by PCR.

F2620

• Re-making overnight to sequence; last night's sample was lost.

Primer order

• TCAGCGAT ATGCAT TATAAACGCAGAAAGGCCCAC, E0240_T9002_rev

• 8-merR.NotI.EcoRI.CAGAAATCATCCTTAGCGAAAGC, BB_P_Rev_Eco
• 8-merF.NotI.SpeI.TCACACAGGAAAGTACTAGATGCG, BB_E0240_Fwd_Spe
• 8-merF.NotI.XbaI.TCACACAGGAAAGTACTAGATGCG, BB_E0240_Fwd_Xba
• 8-merR.NotI.SpeI.GTGCTCAGTATCTCTATCACTGATAGG, BB_R_Rev_Spe
• 8-merR.NotI.EcoRI.GTGCTCAGTATCTCTATCACTGATAGG, BB_R_Rev_Eco
• 8-merF.NotI.XbaI.ATGCGTAAAGGAGAAGAACTTTTC, BB_I2055_Fwd_Xba
• 8-merR.NotI.EcoRI.TTTATTCGACTATAACAAACCATTTTC, BB_T9002_Rev_Eco
• 8-merF.NotI.EcoRI.TCACACAGGAAAGTACTAGATGCG, BB_T9002_Fwd_Pst
• CTTAGTAG.CAATTG.tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactag.ATGCGTAAAGGAGAAGAACTTTTC , I2055_promoter_F
• TCAGCGAT.ATGCAT.TATAAACGCAGAAAGGCCCAC, E0240_R
• 8-merF.NotI.XbaI.ATGGCTTCCTCCGAAGACG, BB_I2056_Fwd_Xba
• 8-merF.NotI.SpeI.TCACACAGGAAAGTACTAGATGGC, BB_I2057_Fwd_Spe
• 8-merF.NotI.XbaI.TCACACAGGAAAGTACTAGATGGC, BB_I2057_Fwd_Xba
• also need to make ccdB primers...