- Mini-prepping o/n's from yesterday.
- Ligation re-plating:
- No growth on I2056-1AC3,1AT3.
- Small colonies on I2057
- Running gel of colony PCR of I2055, and phusion PCR of E0240.
- Order: ladder, E0240, p1010, I2055 (1-5)
- Ran out of DNA for the T9002-3K3 sequencing reaction. Growing another culture to re-sequence tomorrow (with F2620 primers)