Registry/Measurement kit/Notebook/2007-6-27

To Do

• Colony verification PCR of rfp-based RBS tester (done)
• Scarring PCR of E0240 with Tm of 49.5 deg (done)
• Grad. PCR of I2055 (done)
• Decide what to do with rfp promoter tester (ligation)

Colony PCR

• Colony 1 is from yesterday's single colony; 2-7 are from today's

I2055 gradient PCR

• Using another stock of primers
• Using same gradient, 49-60 deg
• Made new dilution of dNTP's

Gel

• row 1: ladder, grad 1-12, ladder
• row 2: ladder, col 1-7, E0240, ladder
  • No bands. Will upload picture.

RFP Promoter tester

• Ligated B0032, J04650, tet and chlor backbones
• Transformed in MG1655