Registry/Measurement kit/Notebook/2007-6-25

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  • Made glycerol stocks of backbone culture
  • Transformed ligated rfp-based devices into MG1655
    • Incubated for 2h to develop tet or chlor resistance
  • Ran scarring PCR of I2055
    • Use spreadsheet to calculate primer dilutions
  • Began digest of backbones, will run overnight