Registry/Measurement kit/Notebook/2007-6-21

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"Scarring" PCR

  • Began PCR to remove BioBrick restriction sites from T9002 and E0240

Sequencing of I2055

  • 2µL of 1.6µM primers
  • needed 200-500 ng of DNA, or about 1µL
  • add water to 12µL.
  • tube 1: forward primers; tube 2: reverse

RFP-based device construction

  • started digests of backbones 1AK3, 1AT3, 1AC3, and parts B0032, R0040, and J04650