Registry/Measurement kit/Notebook/2007-6-21

"Scarring" PCR

• Began PCR to remove BioBrick restriction sites from T9002 and E0240

Sequencing of I2055

• 2µL of 1.6µM primers
• needed 200-500 ng of DNA, or about 1µL
• add water to 12µL.
• tube 1: forward primers; tube 2: reverse

RFP-based device construction

• started digests of backbones 1AK3, 1AT3, 1AC3, and parts B0032, R0040, and J04650