- Add new devices into the Registry
- Check re-plating of devices A and C (done. no growth.)
- Create/order primers for device B
- Mini-prep and sequence device B culture (colony 8) and Jason's three cultures (done)
- Spun at 5k for 4.5 min before prepping
- note: accidentally added 250 of P1 to each tube instead of each culture, but reconsolidated into four miniprep columns. We'll see if this affects the results drastically.
- Eluted with 30µL of EB.
- For future reference: when sequencing, should elute with aqueous NaOH solution of pH 8.5
- Will probably need to re-do this...
"Device B" Primers
- CTTAGTAG + CAATTG + tccctatcagtgatagagattgacatc
- TCAGCGAT + ATGCAT + TATAAACGCAGAAAGGCCCAC
Tm ~53 deg