Registry/Measurement kit/Notebook/2007-6-16

• Plates with transformation of ligation product seem to be overgrown.
• Streaking them out on new Tet plates to get single colonies.

• Actually, the problem is most likely that the tetracycline plates didn't work.

• Poured new Tet and Chloramphenicol plates
• Tet: (1 in 1000): added 500µL to 500mL
• Chlor: (1 in 250): added 2.5 mL to 500mL

• Transferring colonies from old plates to new ones using Jason's makeshift protocol:

1. Add 1 mL of LB to the plate
2. Get the LB to cover all of the colonies
3. Pipet, or if you're feeling brave, pour the LB into a tube
4. Plate 50~100µL onto the new plate

• Placed excess of transfer LB in the -4 fridge.