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- All dry items (tips, flasks, tubes, etc.) - 20 minutes steam, 20 minutes drying
- All liquids (plate agar, media, solutions) - 20 minutes steam
- All waste (old plates, etc.) - Must be in bio-hazard bag. 1 hr in big autoclave.
- Wash with dissolved powdered detergent. Rinse with DI water (Milli-Q if you're worried).
- Do not wash out water bottles with detergent!
- Do not wash empty 10% glycerol bottles with detergent!