Protocol used

1. cells induced overnight from stock plate. 
2. pellet for 5minutes at 5600rpm 
3. after pelleting, liquid was removed via flicking and aspirating. 
4. weigh out tyrosinase and dissolve in phosphate buffer 
5. resuspended cell pellet in tyrosinase 
6. incubated on bench top for an hour 
7. pipet out 20ul onto a petri dish and allow to sit for 20min 
8. wash twice with phosphate buffer
9. stain with coomassie
10. wash with phosphate buffer
11. visualize under microscope