Prather:Protocols

Prather Lab Protocols

• Prather Lab New Student Guide (PDF, Updated June 2011)
• Good Laboratory Practice Handout (PDF, Updated 15Mar2007)
• Molecular Biology Protocols (PDF, Updated Jan. 2009)
  • Growing E. coli in liquid culture and on solid media
  • DNA electrophoresis
  • Gel extraction
  • PCR
  • DNA digestion with restriction enzymes
  • DNA ligation reaction
  • Electrocompetent transformations
  • Preparation of freezer stocks
  • Plasmid miniprepping
  • PIPE cloning

• Advanced Molecular Biology Protocols (PDF, Updated Jan. 2009)
  • Preparation of bacterial lysates by freeze-thaw
  • Bradford total protein assay
  • Boiling-lysis preparation of pDNA
  • Sample dialysis
  • Running a protein gel

• Gibson Chew Back and Anneal Isothermal Assembly
• P. putida Protocols (PDF, Updated Feb. 2009)

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