Pig-tailing to reduce stutter

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Pig-tailing is a technique of adding a few extra nucleotides to the 5' end of a reverse primer in order to prevent stuttering of microsatellite and other length polymorphism markers.

There are two options for Pig tailing that I know of, but I am not sure why to use one over the other:



Make sure to attach them onto the 5' end of whichever primer you are "tailing." Make sure this is not the primer with a fluorescent or M13 label on it.

Here is an example of a reverse primer with PigA attached to it: