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| Cell Line |
Species and Type |
Notes
|
| HeLa |
Human Epithelial |
Monolayer line, Passage at 70-80%
|
| HeLa-FP |
Human Epithelial |
Monolayer line, Passage at 70-80%, Requires 400 ng/mL Puromycin and 600 ug/mL G418, Stable expression of Actin-mRFP and Tubulin-GFP
|
| HEK293 |
Human Embryonic Kidney |
Passage at 70-80%
|
| HFF |
Human Foreskin Fibroblast |
Contact inhibited monolayer, Passage at 70-80%
|
| SAOS2 |
Human Osteoblast-like Epithelial |
Monolayer line, Passage at 70-80%
|
| NIH3T3 |
Mouse Fibroblast |
Passage at 70-80%, Do not allow to become confluent as fusion and differentiation will occur
|
| HL-1 |
Mouse Cardiomyocyte |
BEWARE: Requires very specific culture/serum and growth conditions talk to Dr. Pelling first!
|
| C2C12 |
Mouse Myoblast |
Passage at 70-80%, do not allow to become confluent as fusion and differentiation will occur
|
| IMCD |
Mouse Inner Medullary Collecting Duct Epithelial |
Monolayer Line, DMEM:F12, 10%FBS, Passage at 70-80%
|
| CHO |
Chinese Hamster Ovary Epithelial |
F12, 10%FBS, 1% Non-Essential Amino Acids, Passage at 70-80%
|
| MDCK |
Madin Darby Canine Kidney Epithelial |
Passage at 70-80%, Do not allow to become over confluent, Cells detach slowly from surface
|
|
