Pd.primers
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Pete Davenport's Primers
Primer | sequence | PA01 genome position (1st base 3`) | +/- strand | 5`of | 5` mods e.g. "RE" "stuffer" "restriction site" | Tm (Sigma) |
---|---|---|---|---|---|---|
on1 | AAAAGCTTCAGCCGGCATATCTACGATT | 1557488 | + | lasR | HindIII aa AAGCTT | |
on2 | AAGGTACCCACTTGAGCACGCAACTTGT | - | rsaL | KpnI aa ggtacc | ||
on3 | AAGGTACCAGGATTGGCTTATCCCGAAG | 1559025 | + | lasI | KpnI aa ggtacc | |
on4 | AAGAGCTCGTCCGGGGATGCCTGATAG | 1559962 | - | lasI | SacI aa gagctc | |
on5 | AATCTAGAGATGGTCGAACTGGTCGAAT | 3889608 | + | rhlR | XbaI aa tctaga | |
on6 | AAAAGCTTAACTGCAACGCTTTCTCGAT | 3891310 | - | rhlR | HindIII aa aagctt | |
on7 | AAGAGCTCGCAGAGAGACTACGCAAGTCG | 3889029 | + | rhlI | SacI aa gagctc | |
on8 | AATCTAGACACCACAAGAACATCCAGAAGA | 3889986 | - | rhlI | XbaI aa tctaga | |
on9 | AAGGTACCGGAACCAACTGTTCCAGCAT | 2068690 | + | qscR | KpnI aa ggtacc | |
on10 | AAGAGCTCATTGCCATGACCGAATGTTT | 2070518 | - | qscR | SacI aa gagctc | |
on11 | GGTATCGCGGTGAAGATGAT | + | lasR, on1 | 63.8 | ||
on12 | GCCTGCTGCTCTATTTCCAG | - | lasI, on4 | 63.9 | ||
on13 | TATTCGAGCCAGTGCTCCAT | + | rhlI, on7 | 64.6 | ||
on14 | GGTACACCCCAAGTTCAACG | - | rhlR, on6 | 64.1 | ||
on15 | AGCAGCGAGTTGAAGACCAT | + | qscR, on9 | 63.9 | ||
on16 | CTCGAAGATCCGCACGTT | - | qscR, on10 | 64.1 | ||
on17 | AAGTCGACACCACAAGAACATCCAGAAGA | - | rhlI | SalI aa gtcga(c) | ||
on18 | aaggatccATGTTTTGGGGCTGTGTTCT | + | lasI | BamHI aa ggatcc | ||
on19 | aagtcgacGTCCGGGGATGCCTGATAG | - | lasI | SalI aa gtcgac | ||
on20 | aaggatccGCAGAGAGACTACGCAAGTCG | + | rhlI | BamHI aa ggatcc | ||
on |
notes: "5`of" - what is the first gene or other tagged identified sequence (on either strand) in the 3` direction from the primer