PTXB1
From OpenWetWare
Jump to navigationJump to search
Purpose
E. coli cloning vector that creates a C-terminal fusion to an intein domain, which is useful in techniques such as expressed protein ligation.
Procurement
Sold by New England Biolabs.
Map, Sequence and MCS
- Sequence as FASTA
- Sequence in genbank format
- Technical reference including MCS, map, and other information
Use
Clone a gene using appropriate restriction sites into the MCS. Protein expressed from this plasmid in E. coli can be purified on a chitin affinity column. The tag can be cleaved on-column with reducing agents such as DTT or MESNA, leaving a C-terminal reactive thioester. Peptides and proteins with N-terminal Cys residues will react with the thioester to form a native peptide bond. This vector is part of the IMPACT-CN kit:
Safety
N/A
