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Making 2x PLS

  • 1M Tris pH 6.8 1.5ml
  • 10% SDS 6.0ml
  • Glycerol 3.0ml
  • H20 3.0ml
  • Bromophenol blue small dash to color

vortex, aliquot and freeze at -20

When ready to use, thaw and add Beta metcaptoethanol to 10% (volume/volume) Then dilute to 1x with H20 and