IGEM:Peking University/2008/Experiment/PKU 0627 G2 1

TITLE: PCR-pPT-Gal4-Transtart 20ul*6

	Volume (μL)
Template	0.5
dNTP	2
Enzyme	0.5
Buffer	2
F-Primer	0.2
R-Primer	0.2
ddH2O	14

conditions

Temperature	Time Length(0:00:00)
94°C	02:00
94°C	01:00	4 cycles
50°C	02:00	4 cycles
72°C	05:00	4 cycles
94°C	01:00	30 cycles
55.2,55.6,57.6,59.5,61.3,63.9°C	01:00	30 cycles
72°C	03:00	30 cycles
72°C	10:00
4°C	Hold

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Enzyme cutting-T-Gal4-XbaI-20*6 μL

	Volumn(μL)
DNA	2
XbaI	1
M Buffer	2
BSA	2
ddH2O	13

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Enzyme cutting-T-Gal4-SpeI-HindIII-20*4 μL

	Volumn(μL)
DNA	2
SpeI	1
HindIII	1
M Buffer	2
ddH2O	14

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