IGEM:Peking University/2008/Experiment/PKU 0603 G2 1

PCR

TITLE: PCR-Budding Yeast Genome-Gal4-Easytaq 20ul*6

	Volume (μL)
Template （Budding Yeast Genome）	1
dNTP	2
Enzyme	0.5
Buffer	2
F-Gal4-HindIII-ApaI	0.5
R-Gal4-EcoRI-Xhol	0.5
ddH2O	13.5

conditions

Temperature(&&&)	Time Length(0:00:00)
94°C	02:00
94°C	01:00	4 cycles
50°C	02:00	4 cycles
72°C	00:30	4 cycles
94°C	01:00	30 cycles
52.0,53.6,56.1,58.8,60.1,62.0°C	02:00	30 cycles
72°C	04:00	30 cycles
72°C	05:00
4°C	Hold

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TITLE: PCR-Budding Yeast Genome-Gal4-Transtart 50ul*3

	Volume (μL)
Template （Budding Yeast Genome）	1
dNTP	5
Enzyme	1
Buffer	5
F-Gal4-HindIII-ApaI	1
R-Gal4-EcoRI-Xhol	1
ddH2O	36

conditions

Temperature	Time Length(0:00:00)
94°C	02:00
94°C	01:00	4 cycles
50°C	02:00	4 cycles
72°C	00:30	4 cycles
94°C	01:00	30 cycles
54.7,57.4,60.1°C	02:00	30 cycles
72°C	04:00	30 cycles
72°C	05:00
4°C	Hold

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