IGEM:Peking University/2008/Experiment/PKU 0517 G2 1
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Primer design
- Primer 1
- Primer name: F-Gal4-HindIII-Apal
- Sequence(5'to3'):AAAAAGCTTGGGCCCTTTTAAGAGAGGACAGAGAAGCAAGCCTC
- Tm:64.1°C
- Primer 2
- Primer name: R-Gal4-EcoRI-Xhol
- Sequence(5'to3'):GGGGAATTCCTCGAGTGCGGGGTTTTTCAGTATCTACGATTCATT
- Tm:64.0°C
PCR
| Volume (μL) | |
| Template （Budding Yeast Genome） | 1 |
| dNTP | 5 |
| Enzyme | 1 |
| Buffer | 5 |
| MgSO4(For KOD) | 2 |
| F-Gal4-HindIII-ApaI | 1 |
| R-Gal4-EcoRI-Xhol | 1 |
| ddH2O | 34 |
- conditions
| Temperature(&&&) | Time Length(0:00:00) | |
| 94°C | 07:00 | |
| 94°C | 00:30 | 30 cycles |
| 59°C | 00:30 | 30 cycles |
| 55°C | 00:30 | 30 cycles |
| 72°C | 03:00 | 30 cycles |
| 72°C | 07:00 | |
| 4°C | Hold |
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