IGEM:Peking University/2008/Experiment/PKU 0517 G2 1

Primer design

Primer 1

• Primer name: F-Gal4-HindIII-Apal
• Sequence(5'to3'):AAAAAGCTTGGGCCCTTTTAAGAGAGGACAGAGAAGCAAGCCTC
• Tm:64.1°C

Primer 2

• Primer name: R-Gal4-EcoRI-Xhol
• Sequence(5'to3'):GGGGAATTCCTCGAGTGCGGGGTTTTTCAGTATCTACGATTCATT
• Tm:64.0°C

PCR

TITLE: PCR-Budding Yeast Genome-Gal4-K.O.D 50ul

	Volume (μL)
Template （Budding Yeast Genome）	1
dNTP	5
Enzyme	1
Buffer	5
MgSO4(For KOD)	2
F-Gal4-HindIII-ApaI	1
R-Gal4-EcoRI-Xhol	1
ddH2O	34

conditions

Temperature(&&&)	Time Length(0:00:00)
94°C	07:00
94°C	00:30	30 cycles
59°C	00:30	30 cycles
55°C	00:30	30 cycles
72°C	03:00	30 cycles
72°C	07:00
4°C	Hold

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