Packing the Column
- I purchased 210-250 micron sized soda glass beads from Polysciences.
- Column was Econo-Column 1.5 x 10 cm column from Bio-rad with associated flow-adaptor.
- Add glass beads to 10 ml of ethanol, until a slurry.
- Add to column using a glass tip, and
- Run buffer through syringe to purge flow adaptor of air bubbles.
- Twist lock ring to lock/unlock, and slide flow adaptor into column.