OhioMod2013:Methods/Column Prep

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Packing the Column

  • I purchased 210-250 micron sized soda glass beads from Polysciences.
  • Column was Econo-Column 1.5 x 10 cm column from Bio-rad with associated flow-adaptor.
  • See videos: [1] and flow adaptor [2]
  • Add glass beads to 10 ml of ethanol, until a slurry.
  • Add to column using a glass tip, and
  • Run buffer through syringe to purge flow adaptor of air bubbles.
  • Twist lock ring to lock/unlock, and slide flow adaptor into column.