ONP

o-nitrophenol

• Beta-galactosidase cleaves ONPG to produce ONP and galactose.
• The molar extinction coefficient is reported to be either 4500 M^-1cm^-1 [1] or 4900 M^-1cm^-1 [2] (not original references, better references would be preferable).
• Slightly soluble in water (0.21 g/100ml or 2.1mg/mL)

References

1. Skoneczny M and Rytka J. Oxygen and haem regulate the synthesis of peroxisomal proteins: catalase A, acyl-CoA oxidase and Pex1p in the yeast Saccharomyces cerevisiae; the regulation of these proteins by oxygen is not mediated by haem. Biochem J. 2000 Aug 15;350 Pt 1(Pt 1):313-9.
2. Onishi N and Tanaka T. Purification and properties of a novel thermostable galacto-oligosaccharide-producing beta-galactosidase from Sterigmatomyces elviae CBS8119. Appl Environ Microbiol. 1995 Nov;61(11):4026-30. DOI:10.1128/aem.61.11.4026-4030.1995 |

3. Chemical information