Notebook:Barry Canton/PCR boilerplate

Not sure if this is going to be useful but we'll see.

Reaction 1

Reaction Mix (μl)		Thermocycling
H2O	39	95	1min
Buffer	5 Thermo Pol	91	30s
Forward Primer	1.5 R0183rRNAF	60	30s
Reverse Primer	1.5 rRNA-4RTHR1/rRNA-4RTHR2	70	18mins
dNTPS	2	Cycles	25
Template	1 1/100 of template prep	0	0
Polymerase	2 Vent	4oC	1 hr
First two reactions used rRNA-4 as template. Last two used fixed.

Copy this text:

{{PCR reaction| Water= |Buffer= |PrimerF= |PrimerR= |dNTPs= |Template= |Polymerase= |Denaturing Temp= |Denaturing Time= |Denaturing Cycle Temp= |Denaturing Cycle Time= |Annealing Temp= |Annealing Time= |Extension Temp= |Extension Time= |Cycles= |Final Extension Temp= |Final Extension Time= |4CTime= |Notes= }}