Neutral red

Overview

Neutral Red for gHV68 (DW)

Materials

• Neutral Red
• Whatman filter paper
• Bottles

Procedure

Stock Solution:

1. Add 25g neutral red to 2 liters water.
2. Incubate o/n at 56C protected from light.
3. Filter through Whatman filter paper.
4. Aliquot into small bottles and wrap in tin foil.
5. Autoclave.
6. Store stock at RT.

Working solution:

1. Dilute 3 ml stock solution per 100 ml PBS.
2. Filter.
3. Apply to methylcellulose.

Notes

1. 242.5ml PBS + 7.5ml Stock Neutral Red = 250ml Working Stock Neutral Red

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References

Relevant papers and books

Contact

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