Nachury:Protocols

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Protocols

Under (very slow) construction

Cell Lines

  • Establishing stable lines with retrovirus
  • Cloning fluorescent cells by FACS
  • Growing and harvesting cells in large amounts

Extractology

  • Hypotonic/N2 cavitation lysis
  • Testis extract
  • Retinal extract
  • Retinal detergent extract

Protein Biochemistry

  • TAP purification of protein complexes from mammalian cells
  • Silver staining
  • Methanol-Chloroform precipitation of proteins
  • TCA precipitation of proteins

Immunofluorescence

  • General guidelines
  • PFA/Saponin

Lipids

  • Preparation of liposomes
  • Protein-Liposomes interaction assays
  • PIP strip blotting assays (fat blot)

DNA

  • Old-school maxiprep

Microtubules

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  • This page was last edited on 2 November 2011, at 19:37.
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