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Contact Info

Theobald, Tianwen Wang (an artistic interpretation)
  • Theobald Tianwen Wang
  • Lihu Avenue No.1800, School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, P.R.China
  • Email me through OpenWetWare or to address theobaldwang**gmail.com (Please replace ** with @ when you are using it.).

Education

  • Sept. 2003~June 2006, Ph.D. in Biochemical Engineering, East China University of Science and Technology
  • Sept. 2000~June 2003, M.S. in Molecular Biology and Biochemistry, Fujian Agricultural University
  • Sept. 1996~June 2000, B.S. in Microbiology, Henan Agricultural University

Training

  • Jan. 2011~Jan. 2012, Postdoc in National University of Singapore, Singapore;
  • Jan. 2007~Nov. 2008, Postdoc in Tsinghua university, China.

Researches

  • Synthetic biology
  • Bioinformatics-aided protein engineering
  • Enzyme engineering (discovery through screening and metagenomic method, de novo design)
  • Biochemistry studies of enzymes revealed by large scale sequencing

Grants

  • Spatially engineering the metabolic enzymes in L-Phe biosynthesis, funded by Natural Science Foundation of China (PI);
  • The glutathionalytion of alcohol dehydrogenase from Candida utilis under oxidative stress (PI);
  • Construction of L-phe producer strain with RED recombination and Kan/sacB selective marker (PI);
  • Novel technology for Large‐scale expression and purification of non-fusion recombinant protein based on the ELP-Intein system (PI).

Publications

Journal articles

  1. T. Wang, A. Li, G. Du and J. Chen, A simple and universal method to express protein in its unfused form, Protein Pept Lett, In press.
  2. T. Wang, X. Ma, G. Du and J. Chen, Overview of regulatory strategies and molecular elements in metabolic engineering, Mol. Biotechnol.,In press, DOI:10.1007/s12033-012-9514-y.
  3. T. Wang, X. Ma, G. Du and J. Chen, 2012, Available methods for assembling expression cassettes for synthetic biology, Appl. Microbiol. Biotechnol.,93(5):1853-1863. PMID:22311648.
  4. T. Wang,Y. Hu, , X. Liao, G. Du, J. Chen and J. Xu, 2010, Anti-oxidative stress and beyond: multiple functions of the protein glutathionylation, Protein Pept Lett, 17 (10), 1234-44. [Co-1st and -corresponding author], PMID:20394582.
  5. T. Wang, A. Qiu, F. Meng and H. Zhou, 2009, Changing the Metal Binding Specificity of Superoxide Dismutase from Thermus thermophilus HB-27 by a Single Mutation, Mol Biotechnol, 42 (2), 146-53. PMID:19191037.
  6. T. Wang, H. Zhu, X. Ma, Z. Fei, Y. Ma and D. Wei, 2006, Enhancing enzymatic activity of penicillin G acylase by coexpressing pcm gene, Appl Microbiol Biotechnol, 72 (5), 953-8. PMID:16550378.
  7. T. Wang, H. Zhu, X. Ma, Y. Ma and D. Wei, 2006, Structure-based stabilization of an enzyme: the case of penicillin acylase from Alcaligenes faecalis, Protein Pept Lett, 13 (2), 177-83. PMID:16472081.
  8. T. W. Wang, H. Zhu, X. Y. Ma, T. Zhang, Y. S. Ma and D. Z. Wei, 2006, Mutant library construction in directed molecular evolution: casting a wider net, Mol Biotechnol, 34 (1), 55-68. PMID:16943572.
  9. H. Zhou, X. Liao, T. Wang, G. Du and J. Chen, 2010, Enhanced l-phenylalanine biosynthesis by co-expression of pheA(fbr) and aroF(wt), Bioresour Technol, 101 (11), 4151-6. [Co-corresponding author], PMID:20137911.
  10. H. Zhou, X. Liao, L. Liu, T. Wang, G. Du and J. Chen, 2011, Enhanced L-phenylalanine production by recombinant Escherichia coli BR-42 (pAP-B03) resistant to bacteriophage BP-1 via a two-stage feeding approach, J Ind Microbiol Biotechnol, 38 (9), 1219-27. [Co-corresponding author], PMID:21104105.
  11. H. Zhu, T. W. Wang, S. J. Sun, Y. L. Shen and D. Z. Wei, 2006, Chromosomal integration of the Vitreoscilla hemoglobin gene and its physiological actions in Tremella fuciformis, Appl Microbiol Biotechnol, 72 (4), 770-6. PMID:16501972.
  12. X. Ma, W. Zheng, T. Wang, D. Wei and Y. Ma, 2006, Optimization and high-level expression of a functional GST-tagged rHLT-B in Escherichia coli and GM1 binding ability of purified rHLT-B, J Microbiol, 44 (3), 293-300. PMID:16820759.
  13. M. Xingyuan, Z. Wenyun and W. Tianwen, 2006, Leukocyte function-associated antigen-1: structure, function and application prospects, Protein Pept Lett, 13 (4), 397-400. PMID:16712517.
  14. H. Zhu, R. J. Pan, T. W. Wang, Y. L. Shen and D. Z. Wei, 2006, Functional solubilization of aggregation-prone TRAIL protein facilitated by coexpressing with protein isoaspartate methyltranferase, Appl Microbiol Biotechnol, 72 (5), 1033-8. PMID:16575568.
  15. Yuxin Xu, Wenyun Zheng, Tianwen Wang, Ping Wang, Ling Zhu, Xingyuan Ma*. Genetic protein TmSm(T34A) enhances sensitivity of chemotherapy to breast cell lines as a synergistic drug to doxorubicin. Biomed. Pharmacotherapy, In press.
  16. W. Zheng, X. Ma, D. Wei, T. Wang, Y. Ma and S. Yang, 2005, Molecular cloning and bioinformatics analysis of a novel spliced variant of survivin from human breast cancer cells, DNA Seq, 16 (5), 321-8. PMID:16329164.
  17. X. Ma, W. Zheng, D. Wei, Y. Ma, T. Wang, J. Wang, Q. Liu and S. Yang, 2006, High-level expression, purification and pro-apoptosis activity of HIV-TAT-survivin (T34A) mutant to cancer cells in vitro, J Biotechnol, 123 (3), 367-78. PMID:16406157.
  18. X. Ma, W. Zheng, D. Wei, Y. Ma, T. Wang, J. Wang, Q. Liu and S. Yang, 2006, Construction, expression, and purification of HIV-TAT-survivin (T34A) mutant: a pro-apoptosis protein in Escherichia coli, Protein Expr Purif, 47 (1), 36-44. PMID: 16260148.

Paperwatches

http://dx.doi.org/10.1016/j.tibtech.2011.10.001

Linkouts

  • Links to some labs

Protein engineering

  1. David Baker's lab: http://depts.washington.edu/bakerpg/drupal/

Metabolic engineering and synthetic biology

  1. http://keaslinglab.lbl.gov/
  • Online tools
  1. Multiple alignment: http://www.ebi.ac.uk/Tools/msa/clustalw2/
  • Important Journals
  1. Cell: http://www.cell.com
  2. NPG: http://www.nature.com
  3. Science: http://www.sciencemag.org
  4. PNAS: http://www.pnas.org
  5. JBC: http://www.jbc.org
  6. ASM Journals: http://journlas.asm.org

Updated Time

13:18 2012/2/27